Bcr/abl Molecular Testing by Fluorescence in Situ Hybridization in Untreated Adult Acute Leukemia
نویسنده
چکیده
The BCR/ABL detection in uences diagnosis, prognosis and treatment decisions in patients with chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). We studied the feasibility and the sensitivity of LSI BCR/ABL dual-colour, dual-fusion probe as a usual assay for detection BCR-ABL gene fusion in acute leukemia. A total of 3229 successfully hybridized interphase nuclei from 34 newly diagnosed patients with acute leukemias (12 AML and 22 ALL cases) were analyzed. We used interphase uorescence in situ hybridization (FISH) method and LSI BCR/ABL dual-colour, dual-fusion probe (Vysis) for detection of BCR-ABL gene that span the common breakpoints of t(9;22)(q34;q11.2) and that detect double BCR-ABL fusion in bone marrow cells with this translocation, one on the abnormal chromosome 9 and one on the Philadelphia chromosome (Ph chromosome). Fusion BCR-ABL gene was observed in 9 (28.13%) of the cases with successful hybridization and in 6 (18.75%) of them the results had clinical reliability. The aberrant cell lines were of different sizes 53%-96% of cells were positive for BCR-ABL gene. Fusion signals for BCR-ABL were reported in 2 (16.67%) of AML and in 4 (18.18%) of ALL cases. In conclusion, we proved that LSI BCR-ABL dual-colour, dual-fusion probe (Visys) is characterized with high speci city and sensibility.
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Detection of abl/bcr Fusion Gene in Patients Affected by Chronic Myeloid Leukaemia by Dual-Colour Interphase Fluorescence in situ Hybridisation
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